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Protein A-Cys

Protein A-Cys

货号:1001-04
纯度(SDS-PAGE)
价格:
¥ 635
规格:
数量:
- +
技术资料
    Accession
    P02976
    GeneID
    3919448
    Source
    Escherichia coli.
    Molecular Weight
    Approximately 33.5 kDa with a Cys on C-terminus, a single non-glycosylated polypeptide chain containing 297 amino acids.
    AA Sequence
    NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDC
    Protein per Gram Powder
    See Label.
    Concentration for 1 unity OD at 280 nm
    4.42 mg/ml
    Physical Appearance
    Sterile Filtered White lyophilized (freeze-dried) powder.
    Purity
    > 98 % by SDS-PAGE and HPLC analyses.
    Specificity
    The recombinant Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.
    Endotoxin Level
    Less than 0.1 EU/μg of Protein A-Cys as determined by LAL method.
    Formulation
    Lyophilized from additive free solution.
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    - 12 months from date of receipt, -20 to -70 °C as supplied.
    - 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
    Reconstitution
    Dissolve in distilled water or saline.
    Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at -20 °C.
    SDS-PAGE
    Background
    Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E.Coli and functions essentially the same as native Protein A. It consists of 5 IgG-binding domains E-D-A-B-C aligned in series.
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