- Source
- Escherichia coli
- Molecular Weight
- Approximately 12.9 kDa, a single non-glycosylated polypeptide chain containing 114 amino acids.
- Sequence
- NWVNVISDLK KIEDLIQSMH IDATLYTESD VHPSCKVTAM KCFLLELQVI SLESGDASIH DTVENLIILA NNSLSSNGNV TESGCKECEE LEEKNIKEFL QSFVHIVQMF INTS
- Purity
- > 98% by SDS-PAGE and HPLC analyses.
- Biological Activity
- Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.30-2.60 ng/mL. The specific activity of recombinant human IL-15 is > 1.50 × 108 Units/mg, which is calibrated against rHuIL-15 WHO reference reagnt (NIBSC code: 95/554).
- Physical Appearance
- Sterile filtered white lyophilized (freeze-dried) powder.
- Formulation
- Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
- Endotoxin
- Less than 0.01 EU/µg of rHuIL-15 GMP as determined by LAL method.
- Sterility
- Negative.
- Mycoplasma
- Negative.
- Host Cell Protein
- Less than 0.05% when tested by ELISA.
- Host Cell DNA
- Less than 20 ng/mg when tested by qPCR.
- In Vitro Virus Assay
- Negative.
- Reconstitution
- Prior to opening, it is recommended to centrifuge the vial briefly to bring the contents down the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
- Shipping
- The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- A minimum of 12 months from date of receipt, when stored at ≤ -20 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
- Refer to lot-specific CoA for the Expiry Date.
- Usage
- This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory, or further evaluation purposes. NOT FOR HUMAN USE.
- Quality Statement
- The manufacturing and testing of these products comply with ICH Q7 guidelines.
- Background
- Human Interleukin-15 (IL-15) is expressed by the IL15 gene located on the chromosome 4. It shares approximately 97% and 73% sequence identity with simian and murine IL-15, respectively. Both human and simian IL-15 are active on murine cells. IL-15 is secreted by mononuclear phagocytes (and some other cells), especially macrophages following infection by virus. It possesses a variety of biological functions, including stimulating and maintaining of cellular immune responses, especially regulating T and natural killer (NK) cell activation and proliferation. In additionally, it shares many biological properties with IL-2, including T, B and NK cell-stimulatory activities. IL-15 signals through a complex composed of IL-2/IL-15 receptor beta chain. Although IL-15 lacks sequence homology with IL-2, it has recently been shown that both the beta and gamma chains of the IL-2 receptor are utilized for IL-15 binding and signaling. In addition, an IL-15 specific binding protein has also been cloned from a mouse T cell clone.
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